Journal:

Article Title: Expression and regulation in the brain of the chemokine CCL27 gene locus

doi: 10.1016/j.jneuroim.2010.04.019

Figure Lengend Snippet: Digital images at 200 times magnification showing FITC immunofluorescent signals corresponding to CD3 positive cells (A) in the meningeal spaces of the olfactory bulbs of mice sensitized and challenged with albumin from chicken egg (OVA). Images obtained from Hoechst staining (B) were merged with those of CD3 to confirm co-localization of the CD3 signal with nuclear DNA (C).

Article Snippet: Sections were incubated with the same goat anti-mouse CCL27 antibody used in the fresh frozen protocol for 24 hours at 4° C (1:1,000 dilution) in combination with mouse monoclonal anti-NeuN (1:10,000 dilution) or rabbit polyclonal anti-glial fibrillary acidic protein (GFAP) (1:5000 dilution) or anti-CD3 antibody (Serotec; MCA 1477at 1:1000 dilution).

Techniques: Staining

Journal:

Article Title: Expression and regulation in the brain of the chemokine CCL27 gene locus

doi: 10.1016/j.jneuroim.2010.04.019

Figure Lengend Snippet: Digital images at 100 times magnification (A, B, C) or 400 times magnification (D, E, F) of FITC immunofluorescent signals corresponding to CD3 positive cells (A, D) in the olfactory bulbs of mice sensitized and challenged with albumin from chicken egg (OVA). Images obtained from Hoechst staining (B, E) were merged with those of CD3 to confirm co-localization of the CD3 signal with nuclear DNA (C, F). Arrows indicate some identified CD3 positive cells in the glomerular cell layer (Gl). EPl: external plexiform cell layer; Mi: mitral cell layer; IGr: internal granular cell layer.

Article Snippet: Sections were incubated with the same goat anti-mouse CCL27 antibody used in the fresh frozen protocol for 24 hours at 4° C (1:1,000 dilution) in combination with mouse monoclonal anti-NeuN (1:10,000 dilution) or rabbit polyclonal anti-glial fibrillary acidic protein (GFAP) (1:5000 dilution) or anti-CD3 antibody (Serotec; MCA 1477at 1:1000 dilution).

Techniques: Staining

Journal:

Article Title: Expression and regulation in the brain of the chemokine CCL27 gene locus

doi: 10.1016/j.jneuroim.2010.04.019

Figure Lengend Snippet: Digital image of double fluorescent immunohistochemistry of a CD3 positive cell (FITC, green) in close proximity of CCL27 positive (Cy3, red) cells in the glomerular cell layer of the olfactory bulbs of mice sensitized and challenged with albumin from chicken egg (OVA). Sections were counter stained with Hoechst to reveal nuclear DNA (blue). Scale bar 10 μm.

Article Snippet: Sections were incubated with the same goat anti-mouse CCL27 antibody used in the fresh frozen protocol for 24 hours at 4° C (1:1,000 dilution) in combination with mouse monoclonal anti-NeuN (1:10,000 dilution) or rabbit polyclonal anti-glial fibrillary acidic protein (GFAP) (1:5000 dilution) or anti-CD3 antibody (Serotec; MCA 1477at 1:1000 dilution).

Techniques: Immunohistochemistry, Staining

Journal: NPJ Vaccines

Article Title: A KIF20A-based thermosensitive hydrogel vaccine effectively potentiates immune checkpoint blockade therapy for hepatocellular carcinoma

doi: 10.1038/s41541-024-01060-2

Figure Lengend Snippet: a CD80 and CD86 levels of BMDCs in flow cytometry (The gating strategy was shown in the Supplementary Fig. ). b Quantification of the proportion of CD80 + CD86 + BMDCs. c MHC-I level of BMDCs indicated by flow cytometry (The gating strategy was shown in the Supplementary Fig. ). d Quantification of the proportion of MHC-I + BMDCs. e MHC-II level of BMDCs in flow cytometry (The gating strategy was shown in the Supplementary Fig. ). f Quantification of MHC-II + BMDCs. g CFDA-SE level in CD3 + T cells (The gating strategy was shown in the Supplementary Fig. ). h Quantification of T cell proliferation rate. i CLSM images of infiltrated CD8a + T cells into Hepa1-6 cell spheroids. j Quantification of T cell infiltration.

Article Snippet: The cells were then stained with APC-conjugated anti-mouse CD3 antibody (Elabscience, China), and the CFDA-SE fluorescence intensity of CD3 + cells was analyzed by flow cytometry to assess T cell proliferation rate.

Techniques: Flow Cytometry

Journal: NPJ Vaccines

Article Title: A KIF20A-based thermosensitive hydrogel vaccine effectively potentiates immune checkpoint blockade therapy for hepatocellular carcinoma

doi: 10.1038/s41541-024-01060-2

Figure Lengend Snippet: a Scheme illustration of in vivo treatment and monitoring (Created by the authors with Photoshop v22.5.6). b Bioluminescence images of tumor-bearing mice in different groups. c Relative signal intensity of tumors in different groups. d Body weight of the mice in different groups. e Flow cytometry analyzing CD80 and CD86 in CD11c + dendritic cells in mouse inguinal lymph nodes (Gating strategy was shown in the Supplementary Fig. ). f Quantification of the percentage of CD80 + CD86 + cells in CD11c + dendritic cells. g Flow cytometry analyzing CD8a and CD4 of T cells (CD45 + CD3 + ) in tumor tissues (Gating strategy was shown in the Supplementary Fig. ). h Quantification of the percentage of CD8a + cells in T cells.

Article Snippet: The cells were then stained with APC-conjugated anti-mouse CD3 antibody (Elabscience, China), and the CFDA-SE fluorescence intensity of CD3 + cells was analyzed by flow cytometry to assess T cell proliferation rate.

Techniques: In Vivo, Flow Cytometry